Reaction Details 
Report a problem with these data
Report a problem with these dataCell Reactant:
Transcriptional Regulator TtgR Mutant R176G
Syringe Reactant:
BDBM23446
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
2008-07-23
ΔG°:
-7±0.1 (kcal/mole)
pH:
7.0000±n/a
Log10Kb:
3.8
Temperature:
303.1500±n/a (K)
ΔHobs :
-21.6±0.5 (kJ/mole)
Corrected for ΔHioniz:
no
ΔS° :
-0.05±0 (kJ/mole-K)
Citation
De Lucca, GV; Alguel, Y; Liang, J; Meng, C; De Lucca, I; Terán, W; Krell, T; Ramos, JL; Gallegos, MT; Zhang, X Crystal structures of multidrug binding protein TtgR in complex with antibiotics and plant antimicrobials. J Mol Biol 369:829-40 (2007) [PubMed] ArticleCell React
Source:
Site-directed mutant was cloned, and protein was expressed and purified from E. coli.
Prep. Method:
TtgR mutant protein was further purified by size exclusion chromatography using a Sephacryl HR-200 column (Amersham Biosciences). Eluted fractions of TtgR were pooled, concentrated, and dialyzed against the buffer.
Name:
HTH-type transcriptional regulator TtgR [R176G]
Synonyms:
HTH-type transcriptional regulator ttgR | Toluene efflux pump ttgABC operon repressor | Transcriptional Regulator TtgR Mutant R176G
Type:
Repressor; homodimer
Mol. Mass.:
23752.43
Organism:
Pseudomonas putida (g-Proteobacteria)
Description:
n/a
Residue:
210
Sequence:
MVRRTKEEAQETRAQIIEAAERAFYKRGVARTTLADIAELAGVTRGAIYWHFNNKAELVQALLDSLHETHDHLARASESEDEVDPLGCMRKLLLQVFNELVLDARTRRINEILHHKCEFTDDMCEIRQQRQSAVLDCHKGITLALANAVRRGQLPGELDAERAAVAMFAYVDGLIGRWLLLPDSVDLLGDVEKWVDTGLDMLRLSPALRK
Syringe React
Name:
BDBM23446
Synonyms:
3-(4-hydroxyphenyl)-1-(2,4,6-trihydroxyphenyl)propan-1-one | cid_4788 | CHEMBL45068 | Phloretin
Type:
Dihydrochalcone
Emp. Form.:
TBD
Mol. Mass.:
TBD
SMILES:
TBD
Structure:
