Affinity DataKd: 2.50E+6nMAssay Description:Binding activity towards the active site of macrophage migration inhibitory factor (MIF)More data for this Ligand-Target Pair
Affinity DataKd: 2.40E+4nMAssay Description:Binding affinity to human MIF by fluorescence polarization assayMore data for this Ligand-Target Pair
Affinity DataKd: 111nMAssay Description:Inhibition of recombinant human MIF tautomerase activity assessed as decrease in formation of borate complex of enol product using 4-HPP as substrate...More data for this Ligand-Target Pair
Affinity DataKd: 210nMAssay Description:Inhibition of recombinant human MIF tautomerase activity assessed as decrease in formation of borate complex of enol product using 4-HPP as substrate...More data for this Ligand-Target Pair
Affinity DataKd: 2.31E+4nMAssay Description:Inhibition of recombinant human MIF tautomerase activity assessed as decrease in formation of borate complex of enol product using 4-HPP as substrate...More data for this Ligand-Target Pair
Affinity DataKd: 94nMAssay Description:Inhibition of recombinant human MIF tautomerase activity assessed as decrease in formation of borate complex of enol product using 4-HPP as substrate...More data for this Ligand-Target Pair
Affinity DataKd: 160nMAssay Description:Inhibition of recombinant human MIF tautomerase activity assessed as decrease in formation of borate complex of enol product using 4-HPP as substrate...More data for this Ligand-Target Pair
Affinity DataKd: 350nMAssay Description:Inhibition of recombinant human MIF tautomerase activity assessed as decrease in formation of borate complex of enol product using 4-HPP as substrate...More data for this Ligand-Target Pair
Affinity DataKd: 150nMAssay Description:Inhibition of recombinant human MIF tautomerase activity assessed as decrease in formation of borate complex of enol product using 4-HPP as substrate...More data for this Ligand-Target Pair
Affinity DataKd: 260nMAssay Description:Inhibition of recombinant human MIF tautomerase activity assessed as decrease in formation of borate complex of enol product using 4-HPP as substrate...More data for this Ligand-Target Pair
3D Structure (crystal)
Affinity DataKd: 63nMAssay Description:Inhibition of recombinant human MIF tautomerase activity assessed as decrease in formation of borate complex of enol product using 4-HPP as substrate...More data for this Ligand-Target Pair
Affinity DataKd: 213nMAssay Description:Inhibition of recombinant human MIF tautomerase activity assessed as decrease in formation of borate complex of enol product using 4-HPP as substrate...More data for this Ligand-Target Pair
Affinity DataKd: 5.51E+4nMAssay Description:Binding affinity to human MIF by Trp based fluorescent spectroscopyMore data for this Ligand-Target Pair
Affinity DataKd: 3.59E+4nMAssay Description:Binding affinity to human recombinant MIF expressed in Escherichia coli BL21(DE3) by isothermal titration calorimetric assayMore data for this Ligand-Target Pair
Affinity DataKd: 3.63E+3nMAssay Description:Binding affinity to RED-Tris-NTA-labelled C-terminal His-tagged recombinant human MIF tautomerase activity expressed in Escherichia coli BL21 by micr...More data for this Ligand-Target Pair
Affinity DataKd: 9.04E+3nMAssay Description:Binding affinity to recombinant biotinylated human MIF expressed in Escherichia coli BL21 DE3 by surface plasmon resonance assayMore data for this Ligand-Target Pair
Affinity DataKd: 6.86E+3nMAssay Description:Binding affinity to recombinant biotinylated human MIF expressed in Escherichia coli BL21 DE3 by surface plasmon resonance assayMore data for this Ligand-Target Pair
Affinity DataKd: 1.40E+3nMAssay Description:Binding affinity to recombinant biotinylated human MIF expressed in Escherichia coli BL21 DE3 by surface plasmon resonance assayMore data for this Ligand-Target Pair
Affinity DataKd: 390nMAssay Description:Binding affinity to C-terminal His-tagged recombinant human MIF expressed in Escherichia coli BL21 (DE3) at 100 nM by fluorescence assayMore data for this Ligand-Target Pair
Affinity DataKd: 156nMAssay Description:Binding affinity to C-terminal His-tagged recombinant human MIF expressed in Escherichia coli BL21 (DE3) at 100 nM by fluorescence assayMore data for this Ligand-Target Pair
Affinity DataKd: 16nMAssay Description:Binding affinity to C-terminal His-tagged recombinant human MIF expressed in Escherichia coli BL21 (DE3) at 200 nM by fluorescence assayMore data for this Ligand-Target Pair
Affinity DataKd: 4.70E+4nMAssay Description:Displacement of 4'-(7-Hydroxy-2-oxo-2H-chromen-3-yl)-[1,1'-biphenyl]-4-carboxylic acid from C-terminal His-tagged recombinant human MIF expressed in ...More data for this Ligand-Target Pair
Affinity DataKd: 110nMAssay Description:Displacement of 4'-(7-Hydroxy-2-oxo-2H-chromen-3-yl)-[1,1'-biphenyl]-4-carboxylic acid from C-terminal His-tagged recombinant human MIF expressed in ...More data for this Ligand-Target Pair
Affinity DataKd: 4.00E+3nMAssay Description:Displacement of 4'-(7-Hydroxy-2-oxo-2H-chromen-3-yl)-[1,1'-biphenyl]-4-carboxylic acid from C-terminal His-tagged recombinant human MIF expressed in ...More data for this Ligand-Target Pair
Affinity DataKd: 470nMAssay Description:Displacement of 4'-(7-Hydroxy-2-oxo-2H-chromen-3-yl)-[1,1'-biphenyl]-4-carboxylic acid from C-terminal His-tagged recombinant human MIF expressed in ...More data for this Ligand-Target Pair
Affinity DataKd: 5.60E+3nMAssay Description:Displacement of 4'-(7-Hydroxy-2-oxo-2H-chromen-3-yl)-[1,1'-biphenyl]-4-carboxylic acid from C-terminal His-tagged recombinant human MIF expressed in ...More data for this Ligand-Target Pair
Affinity DataKd: 4.40E+3nMAssay Description:Displacement of 4'-(7-Hydroxy-2-oxo-2H-chromen-3-yl)-[1,1'-biphenyl]-4-carboxylic acid from C-terminal His-tagged recombinant human MIF expressed in ...More data for this Ligand-Target Pair
Affinity DataKd: 1.40E+3nMAssay Description:Displacement of 4'-(7-Hydroxy-2-oxo-2H-chromen-3-yl)-[1,1'-biphenyl]-4-carboxylic acid from C-terminal His-tagged recombinant human MIF expressed in ...More data for this Ligand-Target Pair
Affinity DataKd: 2.90E+3nMAssay Description:Displacement of 4'-(7-Hydroxy-2-oxo-2H-chromen-3-yl)-[1,1'-biphenyl]-4-carboxylic acid from C-terminal His-tagged recombinant human MIF expressed in ...More data for this Ligand-Target Pair
Affinity DataKd: 9.50E+4nMAssay Description:Binding affinity to recombinant human MIF expressed in Escherichia coli BL21 incubated for 10 mins by MST assayMore data for this Ligand-Target Pair
Affinity DataIC50: 6.92nMAssay Description:Assays were performed in small-volume clear-bottom black 96 or 384-well polystyrene plates (Greiner Bio-One). First, 2 μL of the enzyme solution...More data for this Ligand-Target Pair
Affinity DataIC50: 7.24nMAssay Description:Assays were performed in small-volume clear-bottom black 96 or 384-well polystyrene plates (Greiner Bio-One). First, 2 μL of the enzyme solution...More data for this Ligand-Target Pair
Affinity DataIC50: 7.5nMAssay Description:Inhibition of human recombinant MIF tautomeraseMore data for this Ligand-Target Pair
Affinity DataIC50: 9.55nMAssay Description:Assays were performed in small-volume clear-bottom black 96 or 384-well polystyrene plates (Greiner Bio-One). First, 2 μL of the enzyme solution...More data for this Ligand-Target Pair
Affinity DataIC50: 10nMAssay Description:Inhibition of human recombinant MIF tautomeraseMore data for this Ligand-Target Pair
Affinity DataIC50: 10.5nMAssay Description:Assays were performed in small-volume clear-bottom black 96 or 384-well polystyrene plates (Greiner Bio-One). First, 2 μL of the enzyme solution...More data for this Ligand-Target Pair
Affinity DataIC50: 11.2nMAssay Description:Assays were performed in small-volume clear-bottom black 96 or 384-well polystyrene plates (Greiner Bio-One). First, 2 μL of the enzyme solution...More data for this Ligand-Target Pair
Affinity DataIC50: 12.3nMAssay Description:Assays were performed in small-volume clear-bottom black 96 or 384-well polystyrene plates (Greiner Bio-One). First, 2 μL of the enzyme solution...More data for this Ligand-Target Pair
Affinity DataIC50: 12.6nMAssay Description:Assays were performed in small-volume clear-bottom black 96 or 384-well polystyrene plates (Greiner Bio-One). First, 2 μL of the enzyme solution...More data for this Ligand-Target Pair
Affinity DataIC50: 18.6nMAssay Description:Assays were performed in small-volume clear-bottom black 96 or 384-well polystyrene plates (Greiner Bio-One). First, 2 μL of the enzyme solution...More data for this Ligand-Target Pair
Affinity DataIC50: 18.6nMAssay Description:Assays were performed in small-volume clear-bottom black 96 or 384-well polystyrene plates (Greiner Bio-One). First, 2 μL of the enzyme solution...More data for this Ligand-Target Pair
Affinity DataIC50: 20.9nMAssay Description:Assays were performed in small-volume clear-bottom black 96 or 384-well polystyrene plates (Greiner Bio-One). First, 2 μL of the enzyme solution...More data for this Ligand-Target Pair
Affinity DataIC50: 22.9nMAssay Description:Assays were performed in small-volume clear-bottom black 96 or 384-well polystyrene plates (Greiner Bio-One). First, 2 μL of the enzyme solution...More data for this Ligand-Target Pair
Affinity DataIC50: 23.4nMAssay Description:Assays were performed in small-volume clear-bottom black 96 or 384-well polystyrene plates (Greiner Bio-One). First, 2 μL of the enzyme solution...More data for this Ligand-Target Pair
Affinity DataIC50: 25.7nMAssay Description:Assays were performed in small-volume clear-bottom black 96 or 384-well polystyrene plates (Greiner Bio-One). First, 2 μL of the enzyme solution...More data for this Ligand-Target Pair
Affinity DataIC50: 28.8nMAssay Description:Assays were performed in small-volume clear-bottom black 96 or 384-well polystyrene plates (Greiner Bio-One). First, 2 μL of the enzyme solution...More data for this Ligand-Target Pair
Affinity DataIC50: 31.6nMAssay Description:Assays were performed in small-volume clear-bottom black 96 or 384-well polystyrene plates (Greiner Bio-One). First, 2 μL of the enzyme solution...More data for this Ligand-Target Pair
Affinity DataIC50: 36.3nMAssay Description:Assays were performed in small-volume clear-bottom black 96 or 384-well polystyrene plates (Greiner Bio-One). First, 2 μL of the enzyme solution...More data for this Ligand-Target Pair
Affinity DataIC50: 38nMAssay Description:Inhibition of human MIF tautomerase activity expressed in Escherichia coli BL21 (DE3) using 4-hydroxyphenyl pyruvic acid as substrateMore data for this Ligand-Target Pair
