Assay Method Information | |
| ChEMBL_1740515 (CHEMBL4156265) |
Description: | Inhibition of recombinant human N-terminal His6-tagged SIRT5 (34 to 356 residues) expressed in Escherichia coli Transetta(DE3) using GluIQF as substrate preincubated for 30 mins followed by substrate and NAD+ addition measured after 2 hrs by fluorescence assay |
Affinity data for this assay | |
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