| Assay Method Information | |
| | Fluorescence Anisotropy Competitive Binding Assay |
| Description: | Inhibitor binding to JNK3 was assessed by a fluorescence anisotropy competitive binding assay. The reaction mixture (JNK3 and fluorescently labelled inhibitor) is added to wells containing various concentrations of test compound in black 384-well microtitre plates. Fluorescence anisotropy is read in Molecular Devices Acquest (excitation 530 nm/emission 580 nm). |
| Affinity data for this assay | |
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