| Assay Method Information | |
| | Slow-Binding Inhibition Assay |
| Description: | Progress curves for slow-binding inhibition analysis were obtained by adding nitrocefin (final concentration 36 μM) to a solution containing enzyme (0.13 μg/mL) and varying concentrations of the inhibitor thiomaltol in buffer (50 mm MOPS at pH 7.0; final volume 1 mL) in acrylic cuvettes. Hydrolysis of the substrate was monitored for up to 3 min. |
| Affinity data for this assay | |
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