| Assay Method Information | |
| | Cell-Based Functional Assay (5-HT4) |
| Description: | Recombinant CHO cells were established by transfecting an expression vector encoding human 5-HT4a gene and CRE-Luc reporter system. Isolated colonies were screened with 1000 nM 5-HT for the induction of luciferase reporter gene. The clone demonstrating highest 5-HT induced reporter gene was used in the evaluationof the known and novel compounds. Increasing concentrations of the compound were incubated with or without 1000 nM 5-HT for antagonist or agonist mode, respectively, for 5 h in 96 well plate. After incubation, cells were harvested, lysed and reporter gene activity was determined. |
| Affinity data for this assay | |
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