| Assay Method Information | |
| | β-Arrestin2 Assay |
| Description: | β-Arrestin2 translocation was also studied using the PathHunter β-arrestin assay in CHO-K1 cells expressing the KOR (DiscoveRx, Fremont, CA) according to the manufacturer's protocol. Cells were treated with agonist for 90 min prior toassessment of enzyme complementation. For antagonist experiments, the cells were incubated with the antagonist for 60 min prior to agonist addition. Luminescence values were obtained using a Synergy HT luminometer (BioTek, Winooski, VT). Allcompounds were run in parallel experiments in duplicate. |
| Affinity data for this assay | |
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