| Assay Method Information | |
| | ALR2 in vitro Inhibition Assay |
| Description: | The activity of ALR2 enzyme was determined at 340 nm in UV spectrophotometer that depends upon the measurement of NADPH consumption. Each well of the 96-well plate contains 200 µL of assay mixture consisting of potassium phosphate buffer 100 mMat 6.2 pH, 20 µL of 10 mM test sample, 70 µL of enzyme preparation and substrate D,L-glyceraldehyde (40 µL), all mixture was incubated at 37 °C for 5 min and then 0.1 mM NADPH (50 µL) was added as a cofactor in the enzyme reaction and read was measured at 340 nm and followed by incubation at 37 °C for 10 min and thenabsorbance was measured in Eliza plate reader at respective wavelength. Negative and positive control used were 10 m M buffer (20 µL) and 10 mM sulindac (20 µL) [Med. Chem. Commun., 5:1371-1380]. |
| Affinity data for this assay | |
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