| Assay Method Information | |
| | Radiometric Calcium Assay |
| Description: | Radiometric calcium assay was performed as described above with some minor modifications. Assay was carried out in total volume of 200 μl at 30° C. (for TRPV1), 32° C. (for TRPV3) and 25° C. (for TRPV4 and TRPM8). Agonists used for selectivity screening of the different TRPs are: Capsaicin for TRPV1,2-aminoethoxydiphenyl borate for TRPV3, 4α-phorbol 12,13-didecanoate for TRPV4 and Icilin for TRPM8. After 2-5 minutes of agonist treatment, drug was washed and lysed in lysis buffer as mentioned above. Radioactivity in the lysate was measured in Packard Top count after addition of liquid scintillant. |
| Affinity data for this assay | |
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