| Assay Method Information | |
| | Biacore Binding of DAAO Inhibitors |
| Description: | Binding affinity and kinetics were measured using biotinylated recombinant human DAAO bound to a Neutravidin surface in a Biacore binding assay. A custom Neutravidin surface was generated using standard amine coupling methods from Biacore on a CM5 sensor chip. An enzymatically biotinylated human DAAO was immobilized onto the Neutravidin surface to a level of ~9000 Ru. Binding studies were performed in a Biacore 3000 (GE Healthcare) instrument. Compounds were injected at three concentrations, and binding responses were processed using Scrubber 2 (BioLogic Software, Inc.) to align and double reference the data. The kinetic data were fit globally to a simple 1:1 interaction model using Biaeval (GE Healthcare) to obtain the rate constants and affinity. |
| Affinity data for this assay | |
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