| Assay Method Information | |
| | KAT6B Enzyme Activity Assay |
| Description: | a. 1× buffer 2: 50 mM Tris-HCl, pH 7.8; 0.1 mM EDTA; 0.01% v/v tween-20; 1 mM DTT; 0.01% m/v bovine serum albumin.b. KAT6B enzyme solution: 3 nM (final concentration), prepared in buffer 2.c. Mixed substrate of Ac-CoA and H3: a mixed substrate of 30 nM (final concentration) Ac-CoA and 30 nM (final concentration) H3, prepared in buffer 2.d. Compounds: initial concentration of 10 mM, 4-fold dilution, 10 gradient concentrations. All compound concentrations were diluted 2500-fold with buffer 2 for later use.e. Assay reagent: 8 ng/μL (final concentration) AlphaScreen protein A acceptor beads, 8 ng/μL AlphaScreen streptavidin donor beads, 1:1000 diluted acetylated-lysine antibody, and 100 μM anacardic acid; prepared in buffer 2. |
| Affinity data for this assay | |
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