| Assay Method Information | |
| | Inhibitory Effect on the Activity of Isoenzymes (CYP1A2, CYP2C9, CYP2C19, CYP2D6 and CYP3A4) of Human Liver Microsomal Cytochrome P450 |
| Description: | A total of 5 specific probe substrates of 5 isoenzymes of CYP, namely phenacetin (CYP1A2), diclofenac (CYP2C9), (S)-mephenytoin (CYP2C19), dextromethorphan (CYP2D6) and midazolam (CYP3A4) are each co-incubated with human liver microsomes and test compound, and then reduced nicotinamide adenine dinucleotide phosphate (NADPH) was added to initiate the reaction. After the reaction was completed, samples were treated, and the concentrations of 5 metabolites (acetaminophen, 4′-hydroxydiclofenac, 4′-hydroxymephenytoin, dextrorphan and 1′-hydroxymidazolam) generated by the specific substrates were quantitatively detected by LC-MS/MS to calculate the corresponding half inhibitory concentrations (IC50). |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |