| Assay Method Information | |
| | TAK1 Biochemical Assay (1mM ATP) |
| Description: | Assay kits from Promega Corporation V4089 were used according to manufacturer's instructions and adapted as outlined here. ADP-Glo Kinase Assay kit contains ADP-Glo Reagent, Kinase Detection Reagent, kinase detection buffer, ATP, and ADP. The Kinase Enzyme Systems include human recombinant TAK1-TAB1 Kinase (TAK1 (1-303) and TAB1 (437-end)), Native Swine Myelin Basic Protein (MBP) substrate, 5×kinase reaction buffer, and DTT, 1mM ATP. |
| Affinity data for this assay | |
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