Assay Method Information | |
| ChEMBL_2363538 |
Description: | Inhibition of N-terminal 6His/SUMO fused human Sirt2 (38 to 356 residues) expressed in Escherichia coli BL21 using acetyl-H3K9 as substrate preincubated for 15 mins in presence of NAD followed by substrate addition and measured after 5 mins by reverse phase HPLC analysis |
Affinity data for this assay | |
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