| Assay Method Information | |
| | Activity Assay |
| Description: | In an effort to biochemically and structurally characterize human HYPDH, we have evaluated numerous expression constructs (>15) in Escherichia coli with N- and C-terminal truncations. These constructs exhibit different levels of protein production, solubility (i.e., inclusion body formation), FAD+ cofactor loading, and enzymatic activity. Only the constructs containing the residues 147-515 and 156-515 were >96% loaded with FAD+ and active. |
| Affinity data for this assay | |
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