| Assay Method Information | |
| | Enzyme Immunoassay (EIA) Assay |
| Description: | I. Assay Solutions a. Preparation of 0.1M K2HPO4/KH2PO4 buffer (pH 7.4) Prepare 0.1 M KH2PO4 from 1M KH2PO4 (Sigma, Cat# P-8709) Prepare 0.1 M K2HPO4 from powder of K2HPO4 (Fisher, BP363-500) Mix 0.1 M K2HPO4 with 0.1 M KH2PO4 to adjust pH to 7.4. b. Preparation of 0.5% γ-globulin Add 0.1 g of γ-globulin (Sigma, Cat# G-5009) to 20 mL 0.1 M K2HPO4/KH2PO4 buffer (pH 7.4) and make 1-mL/vial aliquots and store in −80° C. c. Preparation of 100 mM GSH Add 307 mg of GSH (Sigma, Cat# G-6529) to 10 mL 0.1 M K2HPO4/KH2PO4 buffer (pH 7.4) and store at −80° C. d. Preparation of Reaction buffer: 198 mL of 0.1M K2HPO4/KH2PO4 buffer (pH 7.4) 2 mM GSH Prepared from 100 mM GSH 0.4 g Glycerol 2 mL of 0.5% γ-globulin Add 0.4 g of glycerol and 2 mL of 0.5% γ-globulin to 198 mL of 0.1 M K2HPO4/KH2PO4 buffer (pH7.4). |
| Affinity data for this assay | |
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