| Assay Method Information | |
| | Enzymatic Assay |
| Description: | Briefly, the enzymatic activity of the PTP1B catalytic domain was determinedat 30°C by monitoring the hydrolysis of pNPP. Dephosphorylation of pNPP generates the product pNP, which was monitored at an absorbance of 405 nm using an EnVision multilabel plate reader (PerkinElmer Life Sciences, Boston, MA, USA). Assays were performed in a total volume of 100 μL containing 50 mmol/L 3-[N-morpholino]propane-sulfonic acid (MOPs), pH = 6.5, 2 mmol/L pNPP, and 30 nmol/L recombinant PTP1B, as well as the indicated concentrations of the inhibitor. |
| Affinity data for this assay | |
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