| Assay Method Information | |
| | Kinetic Assay |
| Description: | To characterize the type and extent inhibition of papain by fukugetin, papain (0.04 nM) was incubated with four concentrations of fukugetin (1.5, 3, 6 and 12μM). For cruzain, enzyme concentration was 12 nM and inhibitor concentrations varied from 1 μM to 10 μM. Analysis of enzyme kinetic data was carried out using the Lineweaver-Burk plot30 in Grafit 5.0 software. Hydrolytic activity was measured immediately 3 h after, mixing fukugetin and enzymes by addition growingconcentrations of Cbz-Phe-Arg-MCA (0.5-22 μM). |
| Affinity data for this assay | |
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