| Assay Method Information | |
| | Tyrosinase Inhibition Assay |
| Description: | Briefly, mushroom tyrosinase (1250 units/mL) and 2 mM L-tyrosine (0.07 mL) were added to a solution of phosphate buffer (0.1 M, pH 6.8, 0.09 mL) containing the test sample. The test mixture (0.2 mL) was incubated for 10 min at 37°C and the absorption due to the formation of dopachrome was monitored at 475 nm. The same mixture except for the plant extract was used as a control. Arbutin (hydroquinone-O-β-glucopyranoside) was used as a positive control. Each treatment was replicated three times. |
| Affinity data for this assay | |
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