| Assay Method Information | |
| | [3H]8-OH-DPAT Binding Assay |
| Description: | For the binding assay, aliquots of receptor membranes, 0.25 nM [3H]8-OH-DPAT (8-hydroxy-2-(di-N-propylamino)tetralin, 154.3 Ci/mM; New England Nuclear, Boston, MA) and appropriate concentrations of test compounds were added to 0.25 mL of 50 mM Tris-HCl (pH 7.4) buffer containing 10 mM MgSO4, 0.5 mM ethylenediaminetetraacetic acid (EDTA), and 0.1% ascorbic acid. Incubations were carried out for 60 min at 27 °C, and these were terminated by rapid filtration using a MicroBeta FilterMate-96 harvester (PerkinElmer) through a Filtermat A glass fiber filter presoaked in 0.3% polyethylenimine (PEI). The filter was washed with ice-cold 50 mM Tris-HCl buffer solution (pH 7.4), and was then covered with MeltiLex, sealed in a sample bag, and dried in an oven. |
| Affinity data for this assay | |
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