| Assay Method Information | |
| | In Vitro AChE/BChE Inhibition Assay |
| Description: | The test was run as described in the following procedure: stock solution of the test compounds was diluted in phosphate buffer pH 7.4 to give final concentrationsbetween: 0.1 nM and 2400 nM. AChE and BChE were dissolved in phosphate buffer pH 7.4 to give 2 U/ml and 4 U/ml, respectively. Furthermore, the acetylthiocholine (ATCh) iodide solutions 1 mM and 2 mM for AChE assay and BChE assay were prepared, respectively. The test medium contained DTNB solution 0.4 mg/ml (76 μl), AChE solution 2 U/ml or BChE solution 4 U/ml (10 μl) respectively, various concentrations of the tested compounds (14 μl) and ATCh iodide solutions 1 mM or 2 mM (40 μl) for AChE or BChE, respectively. The medium was mixed and absorption at412 nm was measured after 10 minutes for AChE and after 8 minutes for BChE at room temperature. Each experiment was performed in triplicate. For the reference value, 14 μl of the test compound solutions were replaced by phosphate buffer pH 7.4. |
| Affinity data for this assay | |
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