| Assay Method Information | |
| | Amoxapine and Protriptyline Inhibition Assay |
| Description: | Reaction conditions were as follows: SULT1A1 or 2A1 (50 nM), PnP (3.0 or 100 uM, respectively; 2 x Km PnP), amoxipine or protriptyline (0, 50, 100, or 300 uM), PAPS (125 uM or 3.0 mM, respectively; >250 x Km PAPS), MgCl2 (5.0 mM), KPO4 (50 mM), pH 7.5, 25 +/- 2 ° C. Sulfation of PnP was monitored optically at λ = 415 nm (ε415 = 10.6 mM-1 cm-1 at pH 7.5 [Whittemore et al., Biochemistry, 24:2477-2482). |
| Affinity data for this assay | |
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