| Assay Method Information | |
| | Spectrophotometric Assay |
| Description: | All compounds were dissolved in DMSO (final DMSO concentration in test solution was 2.0%). Phosphate buffer, pH 6.8, was use to dilute the DMSO stock solution of test compounds. Thirty units of mushroom tyrosinase (0.5 mg/mL) were first pre-incubated with the compounds, in 50 mM phosphate buffer (pH 6.8), for 10 min at 25°C, followed by addition of L-DOPA (0.5 mM). The enzyme reaction was monitored by measuring the change in absorbance at 475 nm of formation of the DOPA chrome for 1 min. The measurement was performed in triplicate for each concentration and averaged before further calculation. |
| Affinity data for this assay | |
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