| Assay Method Information | |
| | Binding Assay |
| Description: | Binding assay using temperature-dependent circular dichroism (TdCD). In TdCD, the loss of protein secondary structure was monitored as a function of temperature. Proteins bound with ligand become more resistant to thermal unfolding compared to the unliganded protein because of the additional stabilization interactions created between the ligand and protein. |
| Affinity data for this assay | |
|---|---|
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