Assay Method Information

Assay Name:  Kinase Assay
Description:  The AMPK peptide including the sequence surrounding the phosphorylation site of AMPK (167GEFLRTSCGSP177), was synthesized at the Support Unit for Bio-material Analysis in the RIKEN Brain Science Institute (BSI) Research Resources Center (RRC). ATP consumption was determined by suing a Kinase-Glo Max luminescent kinase assay (Promega) kit, which quantifies the amount of ATP in the reaction solution.
Affinity data for this assay
 

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