Assay Method Information | |
| ChEMBL_2210105 (CHEMBL5123054) |
Description: | Inhibition of recombinant human Keap1 Kelch domain (321 to 609 residues) expressed in Escherichia coli BL21 (DE3)pLysS cells/Nrf2 (unknown origin) protein-protein interaction using FITC-LDEETGEFL-NH2 as fluorescent substrate preincubated with compound for 30 mins followed by substrate addition and measured after 1 hr by fluorescence polarization assay relative to control |
Affinity data for this assay | |
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