| Assay Method Information | |
| | ChEMBL_2184893 (CHEMBL5096975) |
| Description: | Inhibition of N-terminal 6His-tagged full-length H2N-VE(pY)LDLDLD(PEG8)RVD(pY)VVVDQQ-amide-activated SHP2 (247 to 529 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) using DiFMUP as substrate preincubated for 10 to 30 mins with activating peptide prior to compound addition for 30 mins followed by substrate addition and measured for 6 mins by fluorescence based assay |
| Affinity data for this assay | |
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