| Assay Method Information | |
| | ChEMBL_1890988 (CHEMBL4392742) |
| Description: | Inhibition of recombinant human N-terminal GST-tagged BRAF V600E mutant catalytic domain (416 to 766 residues) expressed in baculovirus expression system using human His6-tagged MEK1 (K97R) as substrate preincubated for 20 mins followed by [33P-gamma]ATP addition and measured after 2 hrs by filter-binding assay |
| Affinity data for this assay | |
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