| Assay Method Information | |
| | ChEMBL_1775674 (CHEMBL4232666) |
| Description: | Inhibition of human N-terminal FLAG-tagged PRMT5 (2 to end)/human N-terminal His-tagged MEP50 (2 to end) expressed in HEK293 cells using biotinylated H4 derived peptide as substrate preincubated for 15 mins followed by substrate addition measured after 60 mins in presence of [3H]SAM by liquid scintillation counting |
| Affinity data for this assay | |
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