| Assay Method Information | |
| | Protease Inhibition Assay |
| Description: | All enzyme assays were performed under initial velocity and steady-state conditions. The conditions for the enzyme catalyzed hydrolysis of the cleavage site peptide VSQN-(beta-naphthylalanine)-PIV were established with respect to time and enzyme concentration to yield linear initial velocity data. IC50 is inhibitor concentration, which inhibits 50% peptide cleavage activity of HIV protease. The detection of product was monitored with fluorescence (excitation 270 nm, emission 330 nm). |
| Affinity data for this assay | |
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