| Assay Method Information | |
| | Radioligand Binding Assay |
| Description: | The affinity of compounds for human recombinant GABA (A) receptors was measured by competition binding using [3H]flunitrazepam. Assays were carried out in 96-well plates and incubation time started by the addition of cell membranes. Following incubation, assays were terminated by filtration through GF/B filters. Radioactivity was counted using a liquid scintillation counter. The percentage inhibition of [3H]flunitrazepam binding, the IC50 and the Ki values were calculated using the Activity Base Software Package (ID Business Solutions, Guildford, UK) according to the Cheng-Prusoff equation. |
| Affinity data for this assay | |
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