| Assay Method Information | |
| | Protease Inhibition Assay |
| Description: | A peptide cleavage assay was performed using the icosapeptide H-Arg-Arg-Ser-Asn-Gln-Val-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-Asn-Ile-Gln-Gly-Arg-Arg-OH, and the activity was monitored by HPLC. The cleavage products were analyzed after elution with a water/acetonitrile gradient, and detection at 215 nm. Ro 31-895919 served as a reference compound in this assay with an IC50 of 6.3 nM. |
| Affinity data for this assay | |
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