| Assay Method Information | |
| | Fluorescence Polarization (FP) Competition Assay |
| Description: | IC50 is the concentration of each compound required to inhibit 50% of the binding between the TR LBD and the SRC2-2 peptide using fluorescence polarization (FP) assay. The competition binding experiments were evaluated using PrismPad, and the IC50 values were obtained by fitting the data to an equation, Sigmoidal dose-response (variable slope) or four parameter logistic equation. |
| Affinity data for this assay | |
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