| Assay Method Information | |
| | BACE Electrochemiluminescence (ECL)-Based Assay |
| Description: | HEK293T cells were stably transfected with APP_NFEV. One day after plating, the cells were treated for 20-24 h with a medium containing a compound or 1% DMSO (v/v) as control. Conditioned medium was treated with biotinylated antibody 6E10 (recognizing residues 5-11) and ruthenylated antibody G2-10 (recognizing C-terminal sequence 34-40), followed by the introduction of streptavidin-coated magnetic beads. The resulting solution was incubated overnight and subjected to ECL analysis by IGEN M-384. |
| Affinity data for this assay | |
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