| Assay Method Information | |
| | Biochemical Human PDE10A Activity Assay |
| Description: | Semi Log compound dilutions starting at a final concentration of 50 μM were dispensed into a black 384 well plate alongside, DMSO and an Inhibited control using the Echo Acoustic dispenser. Both Human PDE10A2 and CD73 in a tris-based assay buffer at the final assay concentrations of 0.25 nM and 1 nM respectively, were pre incubated with the compounds for 15 minutes at room temperature, prior to the addition of the substrates, cGMP, and Phosphate Sensor, also diluted in a tris-based assay buffer at the final assay concentrations of 3 μM and 0.9 μM, respectively. The plate was incubated for a further 35 minutes at room temperature before the fluorescence intensity was measured using an optical filter of Ex 430 nm/Em 450 nm on the BMG CLARIOStar Plate Reader. Data was analysed using a 4-parameter fit. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |