| Assay Method Information | |
| | ATX Inhibitory Activity Assay |
| Description: | The biochemical reaction consisted of 50 mM Tris (pH 8.0), 3 mM KCl, 1 mM CaCl2, 1 mM Mg Cl2, 0.14 mM NaCl and 0.1% bovine serum albumin, which was supplemented with 5 nM recombinant rat ATX, 5 μM 18:1 LPC and the test compound (0.1-10 μM). The reaction was stopped at 2 h by the addition of butanol, prior to the RapidFire-MS-based analysis. |
| Affinity data for this assay | |
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