| Assay Method Information | |
| | SERT Binding Affinity Assay |
| Description: | Conditions for SERT binding assay,Receptor Source Human recombinant (CHO cells)Vehicle 1.0% DMSOIncubation Time 1 hIncubation Temperature 25° C.Incubation Buffer 5 mM Tris-HCl, pH 7.4Ligand 2.0 nM [3H]imipramineNon-Specific Ligand 10.0 μM imipramineKd 1.7 nM |
| Affinity data for this assay | |
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