| Assay Method Information | |
| | In-Vitro Assay |
| Description: | THP1 monocytes were differentiated with PMA (100 ng/mL) and incubated at 37 deg C. for 20 hrs in presence of 5% CO2. 2×105 differentiated cells were plated per well of 96 well tissue culture plates. The cells were primed using 500 ng/mL Lipopolysaccharide and incubating for 4 h under the same condition. The cells were then treated with various concentrations of the compounds for 30 min followed by treatment with 5 mM ATP for 1 hr. The supernatants were collected and analyzed by IL-1b (Mabtech Cat #3415-1H-20) or TNF-α (Mabtech; Cat #3510-1H-20) detection kit. The data were analyzed using GraphPad Prism V7.0. Dose Response Curve (DRC) was constructed to determine the IC50 value by fitting percentage cell survival data to the GraphPad Prism using nonlinear regression analysis. |
| Affinity data for this assay | |
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