| Assay Method Information | |
| | Activity Test of Inhibition of Aminopeptidase N of Target Compound In Vitro |
| Description: | Aminopeptidase N interacts with its commercial substrate L-leucyl p-nitroaniline (purchased from Sigma) to produce p-nitroaniline that is absorbed at 405 nm, and the concentration of p-nitroaniline is positively correlated to the enzyme activity. Measure the absorbance at 405 nm, and calculate the inhibition rate based on the absorbance of the inhibitor group and the control group, and calculate the IC50 value. |
| Affinity data for this assay | |
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