| Assay Method Information | |
| | Competitive Radiometric Binding Assay |
| Description: | Binding affinities were also determined by a competitive radiometric binding assay using 2 nM [3H]estradiol as tracer (PerkinElmer, Waltham, MA) and full-length purified human ERα (Pan Vera/Invitrogen, Carlsbad, CA), as reported previously. The RBA values were calculated using the following equation: IC50 estradiol/IC50 compound×100. |
| Affinity data for this assay | |
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