| Assay Method Information | |
| | ROCK1, ROCK2, PRKX, and PKA Inhibition Assay |
| Description: | Compounds as a powder were dissolved in dimethyl sulfoxide to make a 10 mM stock. Compounds were tested in 10-dose IC50 triplicate mode with a 3-fold serial dilution starting at 1 μM. The control compound, staurosporine, was tested in 10-dose IC50 mode with 4-fold serial dilution starting at 20 μM. The HotSpot kit employing 33P-ATP was used with reactions conducted at 10 μM ATP for each tested enzyme. The percent activity relative to DMSO controls for each concentration was then fitted to a curve using GraphPad Prism to determine the IC50. Curve fits were performed where the enzyme activities at the highest concentration of compounds were less than 65%. An IC50 value less than 50.8 μM or higher than 1 μM is estimated based on the best curve fitting available. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |