| Assay Method Information | |
| | Assay for gamma1-containing GABAA Subtypes |
| Description: | The affinity of compounds at GABAA γ1 subunit-containing receptors was measured by competition for [3H]RO7239181 (67.3 Ci/mmol: Roche) binding to membranes from HEK293F cells (ThermoFisher R79007) expressing human (transiently transfected) receptors of composition α5β2γ1, α2β2γ1, α1β2γ1. For better protein expression of the α2 subunit-containing receptors, the 28 amino acid long signal peptide (Metl to Ala28) of the human GABAA α2 subunit was substituted by the 31 amino acid long signal peptide (Met1 to Ser31) of human GABAA α5 subunit. |
| Affinity data for this assay | |
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