| Assay Method Information | |
| | Thallium flux assay |
| Description: | TBDCHO-K1 cells stably expressing human TREK-1 or HEK293 cells stably expressing human TREK-2 are plated in 384-well plates, cultured overnight, loaded with Thallos dye the following day, treated with test compounds or control compound (tert-butyl (3-((4-(benzyloxy)-2-methylphenyl)carbamoyl)-4-chlorophenyl)carbamate) or 0.3% DMSO (vehicle control) for 10 min, and then treated with thallium stimulus buffer to initiate thallium flux. |
| Affinity data for this assay | |
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