| Assay Method Information | |
| | Inhibition of Ovine COX-1 and COX-2 Catalytic Activity Assay |
| Description: | The target compounds were evaluated for their ability to inhibit ovine COX-1 and COX-2 enzyme catalytic activity (percent inhibition at 50 mm, unless otherwise indicated). IC50 values were determined only for compounds that showed a reasonable COX-1 inhibitory activity (>50%) at 50 mm. Each reported IC50 value and the percentage of inhibition (measured at 50 mm as the tested compound concentration) is the average of the results of three separate assays (triplicate). Enzyme inhibition was determined using a colorimetric COX (ovine) inhibitor screening assay kit (Cayman Chemicals, Ann Arbor, MI, USA) following the manufacturer's instructions. Stock solutions of test compounds were dissolved in a minimum volume of DMSO. |
| Affinity data for this assay | |
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