| Assay Method Information | |
| | 5-HT2A Receptor Binding Assay |
| Description: | 5-HT2A Receptor Radioligand Binding. Affinity of the test compounds for the 5-HT2A receptor was determined in radioligand binding experiments with [3H]ketanserin by WuXi AppTec (Hong Kong) Limited, using methods adapted from the literature and under conditions described in Table 2.TABLE 2Assay conditions for 5-HT2A receptor radioligand binding.Receptor Source HEK293 stable cell lineVehicle 1.0% DMSOIncubation Time 1 hIncubation Temperature 25 C.Incubation Buffer50 mM Tris-HCl, pH 7.4Ligand 1 nM [3H]ketanserinNon-Specific Ligand 1 uM ketanserin |
| Affinity data for this assay | |
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