| Assay Method Information | |
| | PDGFRβ HTRF Assay |
| Description: | Stock Solutions:Assay buffer stock solution, contains 50 mM Hepes, 10 mM MgCl2, 1 mM EGTA, and 0.01% Brij-35, 0.01% ovalbumin, 2 mM DTT at pH 7.5, in molecular biology grade water. Store at room temperature.DTT, 2 M in molecular biology grade water, store at −20° C. in aliquots.Ovalbumin, 10% or 100 mg/mL, prepare fresh on experimental day.PDGFRβ, 116 μM (PDGFRb_08 Prep 02), produced at Accelagen. Store at −80° C. in aliquots.TK-biotin peptide, 0.5 μM in molecular biology grade water, store at −20° C. in aliquots.ATP, 100 mM in molecular biology grade water, store at −20° C. in aliquots.HTRF KinEASE-TK kit: Allow the contents of the Cisbio kit to warm up to room temperature before use. This kit contains HTRF detection buffer, TK-Antibody labeled with Eu3+-cryptate, TK-substrate biotin and Streptavidin-XL665.TK Substrate-Biotin, reconstitute 500 μg lyopholized with 574 μL molecular biology grade water to prepare a 500 μM stock; After use, aliquot the rest and store at −20° C.TK Antibody-Cryptate, reconstitute lyophilized with 1 mL of molecular biology grade water (100× solution) then add 99 mL detection buffer to prepare a ready to use TK-antibody-cryptate solution; the concentration of the TK-antibody-cryptate reagent is not known. After use, aliquot the rest and store at −20° C.Streptavidin-XL665, reconstitute 3 mg lyophilized with 3 mL molecular biology grade water to prepare a 1 mg/mL or 16.67 μM stock; MW=60 kDa; After use, aliquot the rest and store at −20° C. |
| Affinity data for this assay | |
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