Assay Method Information | |
| FRET based assay |
Description: | The ProcartaPlex Human p70S6K beta Simplex Kit measures the level of total p70S6K in cell lysates. It is designed to be combinable with other Simplex kits so you can create your own multiplex panel that utilizes Luminex xMAP technology for protein detection/quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size. For preparation of cell lysates, we recommend use of either ProcartaPlex Cell Lysis Buffer (Cat. No. EPX-999999-901) or Cell Extraction Buffer (Cat. No. FNN0011) supplemented with 0.5 M EDTA and Halt Protease and Phosphatase Inhibitor Single-Use Cocktail (100X) (Cat. No. 78440). These buffers are not included with the panel. Depending on your cell system, other buffers may be suitable as well. |
Affinity data for this assay | |
---|---|
If you find an error in this entry please send us an E-mail |