| Assay Method Information | |
| | Inhibition Activity Test of Compound on hERG Ion Channels |
| Description: | The reagents are purchased from Sigma (St. Louis, MO) except NaOH and KOH for acid-base titration. Final concentrations of tested solutions are prepared on the testing day and dissolved in extracellular fluid. The extracellular fluid (mM) contains: NaCl, 137; KCl, 4; CaCl2, 1.8; MgCl2, 1; HEPES, 10; glucose 10; pH 7.4 (NaOH titration). All the test solutions and the control solutions contain 0.3% DMSO. Intracellular fluid (mM) contains: K Aspartate, 130; MgCl2, 5; EGTA 5; HEPES, 10; Tris-ATP 4; pH 7.2 (KOH titration). |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |