| Assay Method Information | |
| | enzyme-inhibition screening assay |
| Description: | The 16 compounds showing substantial inhibition against TcGlcK at compound concentrations of 20 μM were then examined in a confirmatory assay in which the amino sugar analogues were at a concentration of 20 μM and were tested against the assay's only coupling enzyme, G6PDH, to verify enzyme activity; the assay was performed in quadruplicate. Since none of the compounds inhibited G6PDH by more than 20%, they all proceeded as on-target confirmed hits (Table 2). These confirmed hits included representatives from all three monosaccharide scaffolds tested (i.e., D-GlcN, D-ManN, and D-GalN). |
| Affinity data for this assay | |
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