Reaction Details  Report a problem with these data
Cell Reactant:
HIV-1 Protease C Subtype
Syringe Reactant:
BDBM518
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
2004-06-22
ΔG°:
-12.6±n/a (kcal/mole)
pH:
5.0000±n/a
Log10Kb:
7.8
Temperature:
298.1500±n/a (K)
ΔH° :
2.9±n/a (kJ/mole)
ΔHobs :
2.9±n/a (kJ/mole)
Corrected for ΔHioniz:
not known
ΔS° :
0.05±n/a (kJ/mole-K)
Comments:
In displacement titration, inhibitors were injected into the calorimetric cell containing the protein prebound to a weak inhibitor acetylpepstatin.
Citation
 Velazquez-Campoy, A; Vega, S; Freire, E Amplification of the effects of drug resistance mutations by background polymorphisms in HIV-1 protease from African subtypes. Biochemistry 41:8613-9 (2002) [PubMed]  Article
Cell React
Source:
Plasmid-encoded HIV-1 protease was expressed in E. coli cells.
Purity:
99%
Prep. Method:
HIV-1 protease was purified and refolded from E. coli inclusion bodies.
Name:
HIV-1 Protease C Subtype
Synonyms:
n/a
Type:
Protein Complex
Mol. Mass.:
n/a
Description:
n/a
Components:
This complex has 2 components.
Component 1
Name:
HIV-1 Protease C Subtype chain A
Synonyms:
HIV-1 Protease Mutant (Q7K/M36I/S37N/R41K/H69K/L89M) | HIV-1 Protease C Subtype chain B
Type:
Enzyme Subunit
Mol. Mass.:
10772.76
Organism:
Human immunodeficiency virus type 1
Description:
n/a
Residue:
99
Sequence:
PQVTLWKRPLVTIKIGGQLKEALLDTGADDTVLEEINLPGKWKPKMIGGIGGFIKVRQYDQILIEICGKKAIGTVLVGPTPVNIIGRNMLTQIGCTLNF
  
Component 2
Name:
HIV-1 Protease C Subtype chain A
Synonyms:
HIV-1 Protease Mutant (Q7K/M36I/S37N/R41K/H69K/L89M) | HIV-1 Protease C Subtype chain B
Type:
Enzyme Subunit
Mol. Mass.:
10772.76
Organism:
Human immunodeficiency virus type 1
Description:
n/a
Residue:
99
Sequence:
PQVTLWKRPLVTIKIGGQLKEALLDTGADDTVLEEINLPGKWKPKMIGGIGGFIKVRQYDQILIEICGKKAIGTVLVGPTPVNIIGRNMLTQIGCTLNF
  
Syringe React
Source:
Purified from commercial capsules
Prep. Method:
Further purified by HPLC using a semipreparative C-18 reversed-phase column developed with 0-100% acetonitrile in 0.05% TFA
Name:
BDBM518
Synonyms:
Viracept | CHEMBL584 | (3S,4aS,8aS)-N-tert-butyl-2-[(2R,3R)-2-hydroxy-3-[(3-hydroxy-2-methylphenyl)formamido]-4-(phenylsulfanyl)butyl]-decahydroisoquinoline-3-carboxamide | AG-1343 | Nelfinavir
Type:
Small organic molecule
Emp. Form.:
TBD
Mol. Mass.:
TBD
SMILES:
TBD
Structure:
Search PDB for entries with ligand similarity: